DERBY County under-21s lost 2-0 to Sheffield United at the Blades' Shirecliffe training ground yesterday.
Shaun Miller and Dominic Calvert-Lewin scored for United in the Professional Development League Division Two North fixture.
Goalkeeper Adam Legzdins and midfielder James Bailey played 90 minutes for Derby, and defender Mark O'Brien around 80 minutes as they continue their comebacks from injury.
Miller opened the scoring after 13 minutes when his snap-shot from the edge of the box beat Legzdins.
United's second goal came three minutes after the break when Calvert-Lewin curled his shot into the top corner.
The home side had other chances and hit the bar late on.
For Derby, Kurtis Revan's effort was saved and Valentin Gjokaj saw his header blocked.
SHEFFIELD UNITED U21s: Howard; Hodder, Johns, Calvert-Lewin, Kennedy, McGintty, Smith (Banton 88), Cuvelier (Dimaio 62), Miller, Murphy (Cockerline 80), Brandy.
Sub not used: Eastwood.
DERBY COUNTY U21s: Legzdins; Hanson, Gjokaj, O'Brien (Hayes, 83), Hoganson, Revan (Berry, 71), Dales, Bailey, Dawkins, Lowe, Thomas.
Subs not used: Behrens (gk), Rawson, Wassall.
